Library vs. Library

Genotype + Phenotype

A defining feature of the Zafrens approach is the plurality of application that are enabled around one universal workflow.

Inputs: Cells and beads

Output: Multi-omics and longitudinal imaging (or equivalently, genotype and phenotype)

Our unique capability to link library of Perturbations with Phenotype & Genotype in high-throughput fashion at single cell resolution opens up opportunities for scientific discoveries at a scale never seen before!

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Some examples…

Multiple drug discovery steps compressed to one experiment

Input: Reporter cells + Drug bead library

Output: Cell painting; Whole transcriptome sequencing​

HLA independent TCR discovery with target selectivity

Input: Single CD8+ T cells; autologous APCs; peptide beads

Output: Killing (cytox); IFNg; CD69; 41BB; TCRa,b sequence

Bispecific discovery in 3-cell coculture w. killing and cytokines

Input: Single CHO cell; primary CD8 cells; OVCAR3 cells

Output: Killing (cytox); IFNg secretion; GrzB secretion; Bispecific antibody sequence

CAR-T ICD library profiling to find ICDs with killing and less cytokine secretion

Input: Single CAR-T cell from a CAR-ICD library; Tumor cells

Output: Serial killing; Repeat killing; IFNg; PD1; TIM3